Identification and characterization of catA, a mutation causing catalase deficiency in Dictyostelium discoideum.

摘要

Various strains of Dictyostelium discoideum were assayed for catalase activity. We were able to demonstrate the presence of catalase in lysates of all strains tested except one, ts12m. Lysates of this strain did not show any detectable level of catalase. The increased sensitivity of intact ts12m amoebae to hydrogen peroxide was consistent with the catalase deficiency. We followed catalase activity through a developmental time course for the wild type (Ddb) and were able to show its presence throughout development. No catalase activity was detected from ts12m in any stage of development. The growth and development of this acatalasemic strain of D. discoideum was no different than those of the wild type. The mutation, catA, was assigned to linkage group II.